食管癌筛查队列操作流程与建设标准方案探讨

癌症防治工作的重点在于一级预防和二级预防,通过健康教育引起人们对疾病的重视、自觉改变不良行为习惯、主动参加定期体检是防治癌症性价比最高的措施之一。食管癌作为我国疾病负担较重的恶性肿瘤之一,定期筛查和早诊早治是该病防治工作的重点。而队列研究有助于了解食管癌的自然史和危险因素、识别食管癌高危人群。本研究拟从危险因素调查、疾病监测、生物样本信息搜集等方面对食管癌多维动态随访共享队列的建设规范进行探讨,为今后建立规范统一的食管癌筛查队列研究操作流程和建设标准提供参考。     

药用老鹳草原植物分布概述

通过对药用老鹳草常用品种及其同属原植物地区分布的概述，总结了目前国内外对药用老鹳草研究的各品系，为今后规范中药材的使用，以及开发更多的代用品提供相关前期基础。     

清瘟败毒颗粒质量标准的研究

建立清瘟败毒颗粒剂的质量标准.采用TLC方法对处方中的黄芩、黄连进行了定性鉴别;采用HPLC方法测定黄芩苷的含量.色谱柱为KromasilTMC18;流动相为甲醇-水-磷酸(55:50:0.2),流速为1ml·min-1;检测波长280nm;进样量在0.06～0.21μg范围内线性关系良好(r=0.9996);平均回收率为100.2%,RSD=1.21%(n=6).方法准确可靠,重现性好,可作为清瘟败毒颗粒的质量控制方法.     

中医药数据元和数据集标准规范化研究

详细阐述借助EXCEL进行中医药数据元、数据集标准规范化的具体流程,指出其应用能够提高工作效率,降低出错率,为相关研究提供参考。     

Temporal reduction of LATS kinases in the early preimplantation embryo prevents ICM lineage differentiation

Cellular localization of the Yes-associated protein (YAP) is dependent on large tumor suppressor (LATS) kinase activity and initiates lineage specification in the preimplantation embryo. We temporally reduced LATS activity to disrupt this early event, allowing its reactivation at later stages. This interference resulted in an irreversible lineage misspecification and aberrant polarization of the inner cell mass (ICM). Complementation experiments revealed that neither epiblast nor primitive endoderm can be established from these ICMs. We therefore conclude that precisely timed YAP localization in early morulae is essential to prevent trophectoderm marker expression in, and lineage specification of, the ICM.     

Notch Regulates the Generation of Diverse Cell Types From the Lateral Lineage of Drosophila Antennal Lobe

Abstract:

Diverse neuronal cell types arise from the lateral neuroblast in the antennal lobe of Drosophila. The authors show that loss of Notch function from the entire lineage during development leads to an absence of local interneurons (LNs) with a concomitant increase in the number of projection neurons (PNs). The presence of the intracellular domain of Notch was observed within the nucleus of newly born neurons within the neuroblast lineage. This leads to the suggestion that Notch acts in a binary fate decision to determine formation of LNs and PNs, resulting in two distinct hemilineages from the single neuroblast. The observation of nuclear Notch in several neuroblast lineages leads us to speculate that this mechanism is widespread during the developing Drosophila brain.     

注射用三磷酸胞苷二钠有关物质检验方法的建立

目的:建立注射用三磷酸胞苷二钠(CTP-Na2)有关物质检验方法。方法:采用高效液相色谱法,以C18为填充柱,以含反相离子对试剂的磷酸盐缓冲液为流动相[0.02 mol·L^-1磷酸盐缓冲液-乙腈(90∶10)];流速:1.0 mL·min^-1;检测波长:271 nm;柱温:35℃,采用校正因子计算有关物质。结果:CTP-Na 2在0.093~1.860 mg·mL^-1范围内,溶液的浓度与峰面积的线性关系良好,r=0.9997;平均回收率为99.6%(RSD=1.5%,n=9),CTP-Na2对照品溶液在25 h内的稳定性良好(RSD=0.7%);CTP-Na2及主要降解杂质二磷酸胞苷二钠(CDP-Na2)、单磷酸胞苷二钠(CMP-Na2)的方法定量限分别为4.1、2.1、2.8 ng,检出限分别为0.82、0.16、0.22 ng。结论:现行质量标准中有关物质项存在诸多问题,新建的检测方法操作简便、灵敏度高,可用于质量控制。     

Commitment toward the natural T (iNKT) cell lineage occurs at the CD4+8+ stage of thymic ontogeny

T lineage commitment occurs in a discrete, stage-specific manner during thymic ontogeny. Intrathymic precursor transfer experiments and the identification of CD4(+)8+ double-positive (DP), V alpha 14J alpha 18 natural T (iNKT) cells suggest that commitment to this lineage might occur at the DP stage. Nevertheless, this matter remains contentious because others failed to detect V alpha 14J alpha 18-positive iNKT cells that are CD4(+)8+. In resolution to this issue, we demonstrate that retinoic acid receptor-related orphan receptor gamma (ROR gamma)0/0 thymi, which accumulate immature single-positive (ISP) thymocytes that precede the DP stage, do not rearrange V alpha 14-to-J alpha 18 gene segments, suggesting that this event occurs at a post-ISP stage. Mixed radiation bone marrow chimeras revealed that RORgamma functions in an iNKT cell lineage-specific manner. Further, introgression of a Bcl-x(L) transgene into ROR gamma(0/0) mice, which promotes survival and permits secondary rearrangements of distal V alpha and J alpha gene segments at the DP stage, rescues V alpha 14-to-J alpha 18 recombination. Similarly, introgression of a rearranged V alpha 14J alpha 18 transgene into ROR gamma(0/0) mice results in functional iNKT cells. Thus, our data support the "T cell receptor-instructive (mainstream precursor) model" of iNKT cell lineage specification where V alpha 14-to-J alpha 18 rearrangement, positive selection, and iNKT cell lineage commitment occur at or after the DP stage of ontogeny.